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Space Plants Lab

Space Plants Lab

Space Epigenetics 

This project seeks to use an unbiased genome-wide approach to define the changes in DNA methylation that occur in Arabidopsis during spaceflight in order to better understand the spectrum of responses that allow adaptation to the spaceflight environment.  The current study underway in the lab will build on previous research in Epigenetics that compared the methylome of specific plant cell types.  This study contributed to our fundamental understanding of how biology responds and adapts to the spaceflight environment.  Currently, the next study focuses on whether the epigenetic changes induced by spaceflight in one generation of Arabidopsis are inherited in the next.  This study will be house in the Plant Habitat on board the ISS in the near future.  

Revelent Publications

Zhou M, Sng N, LeFrois C, Paul A-L, Ferl R (2019) Epigenomics in an extraterrestrial environment: organ-specific alteration of DNA methylation and gene express elicited by spaceflight in Arabidopsis thaliana.  BMC Genomics 20: 205 (2019)

 space-epigenetics

Sub-orbital Spaceflight

The objective of this project is to use suborbital spaceflights in Virgin Galactic’s SpaceShip Two and other suborbital vehicles to develop an understanding of the genomic and metabolic plant responses upon entry into and recovery from spaceflight. The long term goal of this line of research is a comprehensive appreciation of the biological responses that occur as terrestrial life experiences spaceflight. We are building on our previous genomic work with Arabidopsis plants by imaging GFP-tagged promoters from specific genes indicated as potentially involved in early spaceflight transitions and by fixing plants during suborbital flight for whole genome expression analysis. These data will provide insights into the effects of early stage spaceflight responses so as to begin to round out a more comprehensive understanding of the temporal and transitional plant responses to spaceflight. 

Revelent Publications

Bamsey MT, et al.,  (2014) Flexible imaging payload for real-time fluorescent biological imaging in parabolic,...

Paul A-L, et al., (2011) Parabolic flight induces changes in gene expression patterns...

Virgin-Galactic-SpaceShipTwo

Past Flights

Virgin Galactic December 2018

Blue Origin NS-10 January 2019

Blue Origin NS-12 December 2019

Transgenic Arabidopsis Gene Expression System (TAGES)

Plants experiencing spaceflight are quite normal in appearance but can exhibit growth habits distinctly different from plants on earth. Historically the spaceflight-induced differences were difficult to dissect due to changing hardware and flight conditions.  During the APEX-TAGES experiments, we observed a remarkable number of gene expression changes during spaceflight that are associated with cell wall restructuring, especially in roots. We also observed, with the ABRS/Green Fluorescent Protein (GFP) imaging system, genetically dependent changes in root growth direction, movement and structure. (NASA Profile: TAGES)

Revelent Publications

Paul A-L et al., (2013) Organ-specific remodeling of the Arabidopsis transcriptome in response to spaceflight. BMC Plant Biology. 13:112

Paul A‐L, et al., (2012) Plant growth strategies are remodeled by spaceflight. BMC Plant Biology. 12:232

TAGES-ISA involves experiments that specifically address growth and molecular changes in Arabidopsis that occur during spaceflight, bringing the molecular and genetic tools of Arabidopsis to bear on fundamental questions of root morphology, growth and cell wall remodeling.(NASA Profile: TAGES-ISA)

Revelent Publications

Zhou M, et al., (2019) Epigenomics in an extraterrestrial environment: organ-specific...  BMC Genomics 20: 205 (2019)

Beisel NS, et al., (2019). Spaceflight-induced alternative splicing... Npj Microgravity, 5: 9

TAGES-ISA

Characterizing Arabidopsis Root Attractions (CARA)

The Characterizing Arabidopsis Root Attractions (CARA) experiment looks at mechanisms at the molecular and genetic level that influence the growth of a plant’s roots in the absence of gravity, and how those change with or without light. Researchers expose one set of seedlings to light, keep another set in the dark, and then examine how each environment influences the patterns of root growth.  Some of the plants are also imaged with the Light Microscopy Module on orbit, and at the end of the experiment, all plants are harvested by the astronaut, and preserved for their return to Earth in order to evaluate genes associated with plant responses on orbit. (NASA Profile: CARA)

Relevent Publications 

Ferl RJ, Paul A-L (2016) The effect of spaceflight on the gravity-sensing auxin gradient of roots... Npj Microgravity 2: 15023

Paul A-L, et al., (2017) Genetic dissection of the Arabidopsis spaceflight transcriptome... PLoS One 12: e0180186

CARA-patch

Biological Research In Canisters – 16

Biological Research In Canisters – 16: The impact of spaceflight on Arabidopsis: Deep sequencing and DNA Arrays as Collaborative Readouts of the Transcriptome of Arabidopsis Seedlings and Undifferentiated Cells in Space (BRIC-16-DNA) compares and contrasts the gene expression responses within two forms of Arabidopsis: whole, etiolated seedlings and undifferentiated cells in culture. The comparison of intact plants with cultures of undifferentiated cells shows that cells can detect space flight and gravity in the absence of tissue or organized developmental structures. (Biological Research In Canisters - 16: The Impact of Spaceflight on Arabidopsis: Deep Sequencing and DNA Arrays as Collaborative Readouts of the Transcriptome of Arabidopsis Seedlings and Undifferentiated Cells in Space)

BRIC-16

Biological Research In Canisters (BRIC) – 16 & 17

The Biological Research in Canisters (BRIC) hardware supports a variety of plant growth investigations.  Biological Research In Canisters – 16: The impact of spaceflight on Arabidopsis: Deep sequencing and DNA Arrays as Collaborative Readouts of the Transcriptome of Arabidopsis Seedlings and Undifferentiated Cells in Space (BRIC-16-DNA) compares and contrasts the gene expression responses within two forms of Arabidopsis: whole, etiolated seedlings and undifferentiated cells in culture. The comparison of intact plants with cultures of undifferentiated cells shows that cells can detect space flight and gravity in the absence of tissue or organized developmental structures. (NASA Profile: BRIC 16)

The Biological Research In Canisters-17-1: Undifferentiated Cell development in Arabidopsis plants in Microgravity (BRIC-17-1) investigation focuses on the growth and development of cell cultures in microgravity. Specimens are preserved with a chemical fixative and returned to the ground for post-flight evaluation. (NASA Profile: BRIC 17)

Revelent Publications 

BRIC 16

Paul A-L et al., Spaceflight transcriptomes...(2012) Astrobiology 12(1): 40-56

Zupanska et al., (2013) Spaceflight engages heat shock protein... American Journal of Botany. 100(1):235-248.

BRIC 17

Zupanska AK et al., (2017) ARG1 Functions in the Physiological Adaptation ... Astrobiology 17: 1077-1111

Zupanska AK et al. (2019). HSFA2 Functions in the Physiological Adaptation ... International Journal of Molecular Sciences, 20

BRIC-16

Hypobaric Plant Biology – Molecular Responses of Arabidopsis to Low Atmospheric Pressure

Atmospheric pressure and composition are among the engineering variables considered in the design and construction of spaceflight vehicles and extraterrestrial habitats. Simply put, the costs of maintaining a pressure vessel at one atmosphere have been traded away throughout the history of spaceflight vehicle design and are traded away in future designs – yet the biological impacts of lower atmospheric pressure, especially on plants, is both profound and poorly understood. The objective of this research is to develop a refined understanding of the metabolic processes involved in plant responses and physiological adaptations to low pressure environments relevant to space exploration. The long-term goal of this line of research is a fundamental understanding of low pressure plant biology within exploration vehicles and structures, with a practical goal of producing plants that are specifically designed to thrive in low atmospheric pressures. The essential drivers of this project are the inescapable engineering limitations to producing orbital, lunar or Martian plant growth facilities that contain earth-normal atmospheric pressures, and the knowledge that plants do mount complex, and sometimes unexpected costly metabolic responses to hypobaria.

Revelent Publications

Zhou M, et al., (2017) Dissecting Low Atmospheric Pressure Stress... Frontiers in Plant Science 8: 528

Paul A-L, et al., (2017) Patterns of Arabidopsis gene expression in the face of hypobaric stress. Annals of Botany Plants 9: plx030

NASA Flight Opportunity Program – F104 Starfighter and Zero-G Flights

The Validating Telemetric Imaging Hardware for Crew-Assisted and Crew-Autonomous Biological Imaging project will test biological fluorescent imaging instrumentation for suborbital applications. The specific objective of this experiment is to take fluorescent imaging instrumentation developed for our shuttle and ISS experiments and calibrate the hardware and data collection capabilities to timeframes optimal for parabolic flight and suborbital applications. We  build on extensive insights gained from spaceflight and ISS deployments of similar technologies, along with PI experience on previous parabolic flight campaigns, to address future science and technology deployments in parabolic and suborbital campaigns. This technology has longer term development potential for small satellite and planetary lander applications as well, and speaks directly to aspects of the Nanotechnology Road Map, particularly the development of sensitive, next-generation imagers that can collect data telemetrically, and in real time. Fluorescent protein-based, gene-expression techniques allow direct observation of how biological entities react to the stresses of spaceflight. (Flight Opprotunities Page: Telemetric Biological Imaging)

2013-F104-ALP